What Should Your Bradford Factor Be

How Bradford Reagent Works Bradford reagent: dissolve 100 mg Coomassie Brilliant Blue G-250 in 50 ml 95% ethanol, add 100 ml 85% (w/v) phosphoric acid. Dilute to 1 liter when the dye has completely dissolved, and filter through Whatman #1 paper just before use. The Bradford protein assay is a time-tested colorimetric assay. When the bradford reagent (acidified coomassie
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