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SDS is incompatible with the Bradford assay. If you want to do a protein assay with SDS-containing samples, I suggest you use the BCA assay.

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In addition to previous answers, the differences may arise from interfering substances in your buffers. For instance SDS can artificially increase the signal in the Bradford assay and therefore…

06/09/2007  · Favorite Answer Bradford protein assay is based on the interaction of Coomassie blue with basic and aromatic amino acids – a complex is made …

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